Matt Barr began acting when he was in high school, being an active participant in his high school's drama program, as well as local musical theater productions. He made his film debut during his senior year, appearing in the film Levelland.
Barr made guest appearances in shows such as CSI: Miami, American Dreams and, most notably, the pilot episode of NBC's Medium. In 2005, he took a recurring role in the short-lived series Commander in Chief, followed the next year by a recurring role on One Tree Hill, where he played the stalker to Hilarie Burton's character.
In 2009, after roles in films such as Protecting the King and The House Bunny, Barr joined the CBS miniseries Harper's Island. The following year he joined the cast of the CW's Hellcats, appearing as Dan Patch.
- Levelland (2003)
- Ten Inch Hero (2007)
- Protecting the King (2007)
- Open Your Eyes (2007)
- The House Bunny (2008)
- Pedro (2008)
- Commander in Chief (recurring, 2005)
- One Tree Hill (recurring, 2006-2007)
- Harper's Island (regular, 2009)
- Trauma (recurring, 2009)
- Friday Night Lights (recurring, 2010)
- Hellcats (regular, 2011-present)