Lisa Goldstein spent her childhood in London, as well as in both sides of the United States. After graduating with a music theatre degree from Elon University in 2003, she trained with the B Street Theatre in Sacramento, before working in several regional stage productions.
In 2007 Goldstein appeared in several musicals for Walt Disney World in Florida, and later joined the cast of One Tree Hill as Millie Huxtable, Brooke's assistant. Originally slated to appear only in one episode, the producers made her a regular in the sixth season. In 2008 she made her film debut in the indie Who Do You Love.